ISO/TS 20224-1:2020 分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第1部分：牛DNA检测方法
标准编号：ISO/TS 20224-1:2020
中文名称：分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第1部分：牛DNA检测方法
英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 1: Bovine DNA detection method
发布日期：2020-07
标准范围
本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食品和饲料的牛特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并且可以应用于检测来自牛的牛材料，包括牛磺酸(Bos taurus)和瘤牛(Bos indicus)。该测定还检测野牛（Bison bison）和牦牛（Bos mutus）物种。靶序列是25号染色体中的牛核β肌动蛋白基因的部分片段(即GenBank登录号NC_037352.1)[1][2][3]，其作为每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR分析具有每个反应5个拷贝的绝对检测限，在该浓度下具有≥95%的可重复性（LOD95%）。
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of bovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of bovine material derived from cattle including taurine (Bos taurus) and zebu (Bos indicus). The assay also detects the species bison (Bison bison) and yak (Bos mutus).The target sequence is a partial fragment of the bovine nuclear beta actin gene in chromosome 25 (i.e. GenBank accession number NC_037352.1)[1][2][3], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD95 %).
标准预览图
