ISO 13641-1:2003 水质-生成气体禁止厌氧细菌测定-第1部分：普通试验

标准编号：ISO 13641-1:2003

中文名称：水质-生成气体禁止厌氧细菌测定-第1部分：普通试验

英文名称：Water quality — Determination of inhibition of gas production of anaerobic bacteria — Part 1: General test

发布日期：2003-05

标准范围

ISO 13641-1:2003规定了一种筛选方法，通过测定污水污泥厌氧消化过程中产生的沼气（二氧化碳和甲烷）来评估物质、混合物、地表水、地下水和废水、废水、污泥或其他环境样品的潜在毒性。与好氧微生物相比，厌氧细菌的生长速度要低得多。因此，厌氧法的试验周期比需氧菌法的试验周期长。本方法适用于水溶性或不溶于水的物质，包括挥发性化学品。注意，对于水溶性低的化合物，需要特别小心，在这些情况下，其他方法可能更合适。该方法可提供有用的信息，用于预测试验材料对厌氧消化池中沼气产生的可能影响。例如，只有更长时间的试验才能表明微生物是否能够适应试验材料，或者可能吸附在污泥上的化合物是否能够在比本试验允许的更长时间内积累到有毒浓度，这些试验更接近于模拟工作消化器。根据ISO 13641-1:2003获得的信息也有助于为厌氧生物降解性试验选择合适的初始质量浓度（例如:。g、 ISO 11734）。然而，与ISO 13641-2中描述的方法相比，使用未稀释污泥的ISO 13641-1:2003不太适合测试稀释消化污泥，在该方法中，质量浓度比ISO 13641-2中描述的稀释百倍。

ISO 13641-1:2003 specifies a screening method for assessing the potential toxicity of substances, mixtures, surface waters, groundwaters and wastewaters, effluents, sludges or other environmental samples by determining the production of biogas (carbon dioxide and methane) from the anaerobic digestion of sewage sludge over periods of up to 3 days. The growth rate of anaerobic bacteria is much lower compared with that of aerobic microorganisms. For this reason, the test periods are longer for anaerobic methods than those used for aerobic bacteria.This method is applicable to substances, soluble or insoluble in water, including volatile chemicals.Note that special care is necessary with compounds of low water-solubility, and in these cases, other methods might be more suitable.This method can provide information that is useful in predicting the likely effect of a test material on biogas production in anaerobic digesters. For example, only longer tests, which simulate working digesters more closely can indicate whether adaptation of the microorganisms to the test material can occur or whether compounds likely to be adsorbed onto sludge can build up to a toxic concentration over a longer period than allowed in this test.Information obtained by ISO 13641-1:2003 can also be helpful in choosing suitable initial mass concentrations for anaerobic biodegradability tests (e.g. ISO 11734). However, ISO 13641-1:2003 using undiluted sludge is less suitable for testing dilute digesting sludge than the method described in ISO 13641-2 where the mass concentrations are a hundredfold more dilute.

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