ISO/TS 19006:2016 纳米技术 5 -(和6)- chloromethyl-2,7二氯醋酸二氢荧光素(cm-h2dcf-da) 纳米颗粒诱导的细胞内活性氧(ROS)在RAW 264.7巨噬细胞系生产检测的评估

标准编号:ISO/TS 19006:2016

中文名称:纳米技术 5 -(和6)- chloromethyl-2,7二氯醋酸二氢荧光素(cm-h2dcf-da) 纳米颗粒诱导的细胞内活性氧(ROS)在RAW 264.7巨噬细胞系生产检测的评估

英文名称:Nanotechnologies — 5-(and 6)-Chloromethyl-2’,7’ Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in

发布日期:2016-08

标准范围

ISO/TS 19006:2016描述了如何使用CM-H2DCFDA测定法测试和评估从暴露于纳米物体、纳米颗粒、其聚集体和附聚物的RAW 264.7巨噬细胞中体外ROS生成获得的结果。ISO/TS 19006:2016中的方案仅限于使用24孔板,因此如果要使用其他板,则需要调整体积并验证方案步骤,以确保检测结果的置信度。

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

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