ISO 6866:1985 动物饲料.游离棉酚和总棉酚的测定

标准编号:ISO 6866:1985

中文名称:动物饲料.游离棉酚和总棉酚的测定

英文名称:Animal feeding stuffs — Determination of free and total gossypol

发布日期:1985-11

标准范围

该方法适用于棉籽和棉籽饼。该方法包括在3-氨基丙烷-1-醇存在下提取化合物,使用2-丙醇和己烷混合物测定游离棉酚,或使用二甲基甲酰胺测定总棉酚。使用苯胺转化为棉酚二苯胺,并在最大吸光度波长(435至445 nm之间)处测量吸光度。

The method is applicable to cottonseed and cottonseed meals and cakes. The method consists in extracting of the compound in the presence of 3-aminopropane-1-ol, either with a mexture of 2-propanol and hexane for the determination of free gossypol or with dimethylformamide for the determination of total gossypol. Conversion to gossypol-dianiline using aniline, and measurement of the absorbance at the wavelength of maximum absorbance (between 435 and 445 nm).

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