ISO/TS 21569-6:2016 分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第4部分：cry1Ab/Ac和Pubi-cry DNA序列检测用实时PCR筛选法

标准编号：ISO/TS 21569-6:2016

中文名称：分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第4部分：cry1Ab/Ac和Pubi-cry DNA序列检测用实时PCR筛选法

英文名称：Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 6: Real-time PCR based screening methods for t

发布日期：2016-11

标准范围

ISO/TS 21569-6:20 16规定了用于检测修饰的cry1Ab/Ac基因的DNA序列的程序和用于检测玉米泛素启动子(Pubi)和cry1Ab/Ac基因之间的DNA转换序列的程序。修饰的cry1Ab/Ac基因和Pubi-cry构建体经常在遗传修饰的Bt植物中发现。两种检测方法均基于实时PCR，可用于定性筛选目的。为了鉴定和定量特定的转基因植物（事件），必须进行后续分析。ISO/TS 21569-6:20 16适用于从食品中提取的DNA分析。它还可以适用于从其他产品如饲料和种子中提取的DNA的分析。这些方法的应用需要从相关基质中提取足够量的可扩增DNA。

ISO/TS 21569-6:2016 specifies a procedure for the detection of a DNA sequence of the modified cry1Ab/Ac gene and a procedure for the detection of the DNA transition sequence between the maize ubiquitin promoter (Pubi) and the cry1Ab/Ac gene. The modified cry1Ab/Ac gene and the Pubi-cry construct are frequently found in genetically modified Bt plants. Both detection methods are based on real-time PCR and can be used for qualitative screening purposes. For identification and quantification of a specific genetically modified plant (event) a follow-up analysis has to be carried out.ISO/TS 21569-6:2016 is applicable for the analysis of DNA extracted from foodstuffs. It may also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

标准预览图