ISO/TS 23893-2:2007 水质 对鱼的生物化学和生理学测量 第2部分:乙氧基异吩唑酮脱乙基酶(EROD)的测定
标准编号:ISO/TS 23893-2:2007
中文名称:水质 对鱼的生物化学和生理学测量 第2部分:乙氧基异吩唑酮脱乙基酶(EROD)的测定
英文名称:Water quality — Biochemical and physiological measurements on fish — Part 2: Determination of ethoxyresorufin-O-deethylase (EROD)
发布日期:2007-11
标准范围
ISO/TS 23893-2:20 07规定了一种使用细胞或微孔板荧光法测量鱼肝匀浆的线粒体后级分(EROD活性所在的亚细胞级分)上的乙氧基间苯二酚-O-脱乙基酶(EROD)酶活性的方法。它适用于在自然环境(淡水或盐水)中取样或在实验室暴露于物质或流出物的鱼类。该方法适用于蛋白质的EROD值大于或等于1 pmol/(min mg)。通过使用细胞(试管)程序可以实现更高的灵敏度。
ISO/TS 23893-2:2007 specifies a method for measuring the ethoxyresorufin-O-deethylase (EROD) enzyme activity on a post-mitochondrial fraction of fish liver homogenate (subcellular fraction in which the EROD activity is located) employing a cell or microplate fluorimetric method.It applies to fish that are sampled in their natural environment (fresh water or salt water) or exposed to substances or effluents in a laboratory.This method is applicable for EROD values greater than or equal to 1 pmol/(min mg) of proteins. A higher sensitivity may be achieved by using a cell (test tube) procedure.
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