ISO 20837:2006 食品及饲料的微生物学-食品内含病原体（物）检测用聚合连锁反应-定性方法的样品制备要求

标准编号：ISO 20837:2006

中文名称：食品及饲料的微生物学-食品内含病原体（物）检测用聚合连锁反应-定性方法的样品制备要求

英文名称：Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection

发布日期：2006-04

标准范围

ISO 20837:2006提供了样品制备的标准和示例，以获得PCR兼容的样品或质量和数量适合PCR的核酸。它描述了所涉及的一般原则。附录A中给出了有关微生物富集的标准参考，附录B中给出了DNA提取的详细方法。ISO 20837:2006适用于食品基质，但也可适用于饲料和农业/环境基质，如有必要，可进行一些调整。

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

标准预览图