ISO 10260:1992 水质.生物化学参数测量.a-叶绿素浓缩光谱测定

标准编号:ISO 10260:1992

中文名称:水质.生物化学参数测量.a-叶绿素浓缩光谱测定

英文名称:Water quality — Measurement of biochemical parameters — Spectrometric determination of the chlorophyll-a concentration

发布日期:1992-07

标准范围

规定的程序可用于自然地表水中的浮游植物和生物分析中的藻类生长测试。通过适当的采样,它也可以应用于底栖植物群落。其原理是通过过滤收集藻类,提取藻类色素,光谱法测定提取物中的叶绿素-a浓度,根据提取物酸化前后665 nm处的吸光度差评估叶绿素-a和色素浓度。

The procedure specified can be applied for phytoplankton in natural surface waters and for testing algal growth in bio-assays. Using appropriate sampling it can also be applied to phytobenthic communities. The principle is collection of algae by filtration, extraction of algal pigments, spectrometric determination of the chlorophyll-a concentration in the extract, evaluation of the chlorophyll-a and phaeopigment concentration from the difference of absorbance at 665 nm prior to and after acidification of the extract.

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