ISO 8128-2:1993 苹果汁.浓缩苹果汁和含苹果汁的饮料.展开青霉素含量的测定.第2部分：薄层色谱方法

标准编号：ISO 8128-2:1993

中文名称：苹果汁.浓缩苹果汁和含苹果汁的饮料.展开青霉素含量的测定.第2部分：薄层色谱方法

英文名称：Apple juice, apple juice concentrates and drinks containing apple juice — Determination of patulin content — Part 2: Method using thin-layer chromatography

发布日期：1993-07

标准范围

所述方法的原理是在乙酸乙酯和氯仿（体积比为3:2）的混合物中提取棒曲霉素，在硅胶柱上过滤提取物，并通过双向薄层色谱法进行定性和定量测定。使用3-甲基-2-苯并噻唑啉腙（MBTH）盐酸盐溶液形成斑点。基于50毫升即饮苹果汁，检测限为25μg/l。更精确的分析方法是HPLC。

The principle of the method specified is extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column and qualitative and quantitative determination by means of two-directional thin-layer chromatography. The spots are developed using a 3-methyl-2-benzothiazoline hydrazone (MBTH) hydrochloride solution. The limit of detection is 25 μg/l, based on 50 ml of ready-to-drink apple juice. The more precise method of analysis is HPLC.

标准预览图