ISO 11701:2009 植物的脂肪和油 用高性能液相色谱（HPLC）光散射探测器测定卵磷脂中磷脂含量

标准编号：ISO 11701:2009

中文名称：植物的脂肪和油 用高性能液相色谱（HPLC）光散射探测器测定卵磷脂中磷脂含量

英文名称：Vegetable fats and oils — Determination of phospholipids content in lecithins by HPLC using a light-scattering detector

发布日期：2009-12

标准范围

ISO 11701:2009规定了使用二醇柱和光散射检测器通过高效液相色谱（HPLC）定量测定磷脂含量的方法。该方法适用于原油、含卵磷脂的油，以及来自植物脂肪和油的无油卵磷脂和卵磷脂馏分。由于溶血磷脂酰乙醇胺（LPE）、溶血磷脂酰肌醇（LPI）和溶血磷脂酸（LPA）的峰分离不足，本方法不适用于动物和反刍动物卵磷脂和酶解卵磷脂。

ISO 11701:2009 specifies a method for the quantitative determination of phospholipids content by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector.The method is applicable to crude, oil-containing lecithins, and to oil-free lecithins and lecithin fractions from vegetable fats and oils.The method is not applicable to animal and ruminant lecithins and enzymatically hydrolysed lecithins as the peak separation of lysophosphatidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient.

标准预览图