ISO/TS 4988:2022 纳米技术 使用单细胞生物四膜虫对悬浮液中制造的纳米物体的毒性评估和生物同化

标准编号：ISO/TS 4988:2022

中文名称：纳米技术 使用单细胞生物四膜虫对悬浮液中制造的纳米物体的毒性评估和生物同化

英文名称：Nanotechnologies — Toxicity assessment and bioassimilation of manufactured nano-objects in suspension using the unicellular organism Tetrahymena sp.

发布日期：2022-05

标准范围

本文件提供了一种可靠且可重复的方法，用于使用四膜虫同时评估人造纳米物体（MNOs）的暴露和毒性。摄入的内化物质（MNOs）表示水生暴露。本文件适用于所有研究MNOs纳米（生态）毒性和能够培养四膜虫的中心。该方法使用四膜虫。评估MNO的暴露和影响。此外，对研究MNO与活细胞的生物相互作用感兴趣的中心（实验室）也可以使用该测试。该方法适用于纳米物体，例如纳米颗粒、特定尺寸（微米尺寸范围内）的纳米纤维、纳米板及其聚集体和团聚体。

This document provides a reliable and repeatable method for simultaneous assessment of both exposure and toxicity of manufactured nano-objects (MNOs) using Tetrahymena sp. The ingested, internalized material (MNOs) indicates aquatic exposure.This document is intended to be used by all the centers working with nano(eco)toxicity of MNOs and capable of culturing of Tetrahymena sp. The method uses Tetrahymena sp. to assess exposure and effects of MNOs. In addition, the test can be used by centers (laboratories) interested in investigating the biological interaction of MNOs with living cells.This method is applicable to nano-objects such as nanoparticles, nanofibres of certain size (in a μm size range), nanoplates, as well as their aggregates and agglomerates.

标准预览图