ISO/TS 20224-11:2024 分子生物标记分析 用实时PCR检测食品和饲料中动物源性物质 第11部分：鸽子的DNA检测方法
标准编号：ISO/TS 20224-11:2024
中文名称：分子生物标记分析 用实时PCR检测食品和饲料中动物源性物质 第11部分：鸽子的DNA检测方法
英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 11: Pigeon DNA detection method
发布日期：2024-02
标准范围
本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的鸽子特异性DNA。该方法可用于区分岩鸽（Columba livia）与其他家禽肉（如鹅、鸭、鹌鹑、野鸡）。它需要从相关基质中提取足够量的PCR可扩增DNA。靶序列是Columba livia品种Danish Tumbler未放置基因组支架CLIV_1.0scaffold114(即GenBank登录号NW_004973337.1)[1]的部分片段，其作为每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR测定的绝对检测限为5？每个反应的副本，用？≥？95？该浓度下的置信度%（LOD95？%）。
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of pigeon-specific DNA derived from food and feed. The method can be applied to distinguish rock pigeon (Columba livia) from other domestic poultry meats (e. g. goose, duck, quail, pheasant). It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix.
The target sequence is a partial fragment of Columba livia breed Danish Tumbler unplaced genomic scaffold, Cliv_1.0 scaffold114 (i.e. GenBank accession number NW_004973337.1),[1] which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).
标准预览图
