ISO/TS 20224-9:2022 分子生物标志物分析 用实时PCR检测食品和饲料中的动物源性物质 第9部分：鹅DNA检测方法
标准编号：ISO/TS 20224-9:2022
中文名称：分子生物标志物分析 用实时PCR检测食品和饲料中的动物源性物质 第9部分：鹅DNA检测方法
英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 9: Goose DNA detection method
发布日期：2022-10
标准范围
本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的鹅特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并且可以应用于来自家养品种天鹅（Anser cygnoides domesticus）和家养鹅（Anser anser domesticus）的鹅材料的检测。预计将交叉检测鹅类的短喙鹅、印度鹅、加拿大鹅、阿氏鹅、颊鹅、天鹅座鹅、彩色鹅、耳鹅、牙买加鹅和痣鹅。该方法可用于区分鹅肝最常见的掺假物——家鹅（Anser cygnoides domesticus）和家鹅（Anser anser domesticus）和家鹅（Anser anser domesticus）。[1]它还能够将家鹅与其他高端家禽肉（鹌鹑、鸽子、野鸡）区分开来。靶序列是鹅鹅分离物SCWG-2014品种四川白鹅未放置基因组支架GooseV1.0 scaffold320(即GenBank登录号NW_025927981.1)[2]的部分片段，其作为每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR测定的绝对检测限为5？每个反应的副本，用？≥？95？该浓度下的置信度%（LOD95？%）。
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of goose-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of goose material derived from domestic breeds of swan goose (Anser cygnoides domesticus) and domestic goose (Anser anser domesticus). Cross detection of Anser brachyrhynchus, Anser indicus, Branta canadensis, Cygnus atratus, Cygnus buccinator, Cygnus cygnus, Cygnus olor, Nettapus auritus, Oxyura jamaicensis and Stictonetta naevosa of Anseriformes is expected.The method can be applied to distinguish domestic breeds of swan goose (Anser cygnoides domesticus) and domestic goose (Anser anser domesticus) from domestic chicken, duck and turkey which are the most common adulterants of foie gras.[1] It is also able to differentiate the domestic goose from other high-end domestic poultry meats (quail, pigeon, pheasant).The target sequence is a partial fragment of the Anser cygnoides isolate SCWG-2014 breed Sichuan white goose unplaced genomic scaffold, GooseV1.0 scaffold320 (i.e. GenBank accession number NW_025927981.1)[2], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).
标准预览图
