ISO 5510:1984 动物饲料.有效赖氨酸的测定

标准编号：ISO 5510:1984

中文名称：动物饲料.有效赖氨酸的测定

英文名称：Animal feeding stuffs — Determination of available lysine

发布日期：1984-11

标准范围

该方法包括使用研磨试验部分的盐酸水解，通过柱色谱分离总赖氨酸，并在570 nm处通过光谱测定进行测定。在碱性介质中，第二个研磨试验部分与2,4-二硝基氟苯的乙醇溶液反应，纯化，然后用盐酸水解，通过柱色谱分离不可用的赖氨酸，并在570 nm处通过光谱测定进行测定。

The method consists in hydrolysing with hydrochloric acid of a ground test portion, dollowed by separation of the total lysine by column chromatography and determination by spectrometry at 570 nm. Reaction of a second ground test portion with an ethanolic solution of 2,4-dinitrofluorobenzene in an alkaline medium, purification, then hydrolysis with hydrochloric acid, separation of the non-available lysine by column chromatography, and determination by spectrometry at 570 nm.

标准预览图