ISO 4214:2022 牛奶和奶制品 婴儿和成人/儿童营养配方奶粉和乳制品中氨基酸的测定

标准编号：ISO 4214:2022

中文名称：牛奶和奶制品 婴儿和成人/儿童营养配方奶粉和乳制品中氨基酸的测定

英文名称：Milk and milk products — Determination of amino acids in infant and adult/paediatric nutritional formulas and dairy products

发布日期：2022-12

标准范围

本文件规定了一种使用6-氨基喹啉基-N-羟基-琥珀酰亚胺基氨基甲酸酯（ACQ）衍生，然后超高效液相色谱（UHPLC）分离和紫外（UV）检测定量测定总氨基酸的方法。它规定了在一次分析中测定以下氨基酸的方法:丙氨酸、精氨酸、天冬氨酸（与天冬酰胺组合）、胱氨酸（半胱氨酸的二聚体，与半胱氨酸组合）、谷氨酸（与谷氨酰胺组合）、甘氨酸、组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、脯氨酸、丝氨酸、苏氨酸、酪氨酸和缬氨酸。本方法不适用于色氨酸的测定。该方法适用于婴儿和成人/儿科营养配方奶粉、乳制品和其他基质如谷物。它已在婴儿配方奶粉（牛奶和大豆基，包括部分水解和元素产品）、幼儿配方奶粉、成人营养粉、UHT脱脂牛奶、乳清粉、酪蛋白酸钠、全脂奶粉、麸皮宠物食品、干宠物食品和早餐麦片中得到验证（详见附件？A）。

This document specifies a method for the quantitative determination of total amino acids using 6-aminoquinolyl-N-hydroxy-succinimidyl carbamate (ACQ) derivatization followed by ultra-high-performance liquid chromatography (UHPLC) separation and ultraviolet (UV) detection. It specifies a method for the determination, in one single analysis, of the following amino acids: alanine, arginine, aspartic acid (combined with asparagine), cystine (dimer of cysteine, combined with cysteine), glutamic acid (combined with glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine.

This method does not apply to the determination of tryptophan.

This method is applicable to infant and adult/paediatric nutritional formulas, dairy products and other matrices such as cereals. It was validated in infant formulas (milk- and soy-based, including partially hydrolysed and elemental products), toddler formula, adult nutritional powder, UHT skimmed milk, whey powder, sodium caseinate, whole milk powder, bran pet food, dry pet food and breakfast cereal (see Annex A for details).

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