ISO/TS 21569-2:2021 分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第2部分：构建特定的用于检测亚麻籽和亚麻籽产品中安全性未经审查转基因（FP967）事件的实时性PCR方法

标准编号：ISO/TS 21569-2:2021

中文名称：分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第2部分：构建特定的用于检测亚麻籽和亚麻籽产品中安全性未经审查转基因（FP967）事件的实时性PCR方法

英文名称：Molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 2: Construct-specific real-time PCR method for detection of event FP9

发布日期：2021-07

标准范围

本文件规定了用于检测存在于遗传修饰的亚麻(Linum usitatissimum)品系(事件FP967，也称为“CDC Triffid”)中的DNA序列的程序。为此，将提取的DNA用于实时PCR，并且通过扩增代表来自根癌农杆菌的胭脂碱合酶基因终止子(Tnos)和来自大肠杆菌的1类整合子的二氢叶酸还原酶基因(Dfral)之间的转变的105bp DNA序列来特异性地检测遗传修饰(GM)。所描述的方法适用于从食品中提取的DNA的分析。它还可以适用于从其他产品如饲料和种子中提取的DNA的分析。该方法的应用需要从相关基质中提取足够量的可扩增DNA以用于分析。

This document specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”). For this purpose, extracted DNA is used in a real-time PCR and the genetic modification (GM) is specifically detected by amplification of a 105 bp DNA sequence representing the transition between the nopaline synthase gene terminator (Tnos) from Agrobacterium tumefaciens and the dihydrofolate reductase gene (dfrA1) from a Class 1 integron of Escherichia coli.The method described is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.

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