ISO/TS 15213-3:2024 食物链的微生物学 梭状芽孢杆菌属检测和计数用并行法 第3部分：产气荚膜梭菌的检测

标准编号：ISO/TS 15213-3:2024

中文名称：食物链的微生物学 梭状芽孢杆菌属检测和计数用并行法 第3部分：产气荚膜梭菌的检测

英文名称：Microbiology of the food chain — Horizontal method for the detection and enumeration of Clostridium spp. — Part 3: Detection of Clostridium perfringens

发布日期：2024-05

标准范围

本文件规定了梭状芽孢杆菌（C.）的检测？气味。本文件适用于:—？供人类消费的产品；—？用于动物喂养的产品；—？食品和饲料生产和处理领域的环境样本；—？初级生产阶段的样品。这种水平方法最初是为检查属于食物链的所有样品而开发的。根据本文件发布时可获得的信息，认为该方法完全适用于检查属于食物链的所有样品。然而，由于食物链中的产品种类繁多，这种横向方法可能并不适合所有产品的每个细节。然而，期望所需的修改被最小化，使得它们不会导致与该水平方法的显著偏差。笔记？对以下食品类别进行了少量参与实验室（<10个）的实验室间研究:—？即食、即热肉制品；—？蛋和蛋制品（衍生物）；—？即食、即热渔业产品；—？加工水果和蔬菜；—？婴儿配方奶粉和婴儿谷物（含益生菌）；—？多成分食物或膳食成分。它还在少数参与实验室中对以下其他类别进行了验证:—？环境样品（食品或饲料生产）。由于该方法通常不用于初级生产阶段的样品，因此该类别不包括在实验室间研究中。因此，未获得该类别的性能特征。该方法尚未针对“宠物食品和动物饲料”类别进行验证，因为用于实验室间研究的供试品已经被天然污染。气味。鉴于参与实验室间研究的实验室数量有限，计算的性能特征可用作方法性能的指示值。有关验证的详细信息，请参见条款？11和附件？C到？F.

This document specifies the detection of Clostridium (C.) perfringens.

This document is applicable to:

—     products intended for human consumption;

—     products intended for animal feeding;

—     environmental samples in the area of food and feed production and handling;

—     samples from the primary production stage.

This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.

NOTE          Interlaboratory studies with a small number of participating laboratories (<10) were conducted for the following food categories:

—      ready-to-eat, ready-to-reheat meat products;

—      eggs and egg products (derivates);

—      ready-to-eat, ready-to-reheat fishery products;

—      processed fruits and vegetables;

—      infant formula and infant cereals (with probiotics);

—      multi-component foods or meal components.

It has also been validated with a small number of participating laboratories for the following other category:

—      environmental samples (food or feed production).

Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. The method has not been validated for the category ‘pet food and animal feed’, as the test samples used for the interlaboratory study were already naturally contaminated with C. perfringens. Given the limited number of participating laboratories in the interlaboratory studies, the calculated performance characteristics can be used as indicative values of the method performance. For detailed information on the validation, see Clause 11 and Annexes C to F.

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