ISO 29201:2012 水的质量 试验结果可变性及微生物计数法的测量不确定度

标准编号：ISO 29201:2012

中文名称：水的质量 试验结果可变性及微生物计数法的测量不确定度

英文名称：Water quality — The variability of test results and the uncertainty of measurement of microbiological enumeration methods

发布日期：2012-01

标准范围

本国际标准为微生物定量分析中的不确定度评估提供了指南基于培养物微生物颗粒计数的分析。它涵盖了菌落计数方法的所有变体以及最可能的数字估计。两种方法，组件（也称为自底向上或逐步）和修改的全局（自上而下）方法包括。其目的是明确实验室内操作可变性和组合不确定度的值对最终结果的影响可以获得测试结果。本程序不适用于枚举法以外的方法。注1:大多数附件为规范性附件。但是，仅适用与每种情况相关的附件。如果选择如果是全局方法，则可以跳过属于组件方法的所有规范性附录，反之亦然。注2:源分析前抽样方差不在本国际标准范围内，但需要在抽样设计和监测方案中予以解决。注3:基于分析结果的决策的怀疑或不确定性估计值不在本国际标准的范围内。注4:在能力验证和相互校准方案中观察到的额外分析变化也不存在在本国际标准中有详细说明，但在分析控制中有必要将其考虑在内。用途在不确定度估计中，相互校准数据的差异提供了将实验室之间的偏差包括在内的可能性（Nordtest Report TR 537[12]）。

This International Standard gives guidelines for the evaluation of uncertainty in quantitative microbiologicalanalyses based on enumeration of microbial particles by culture. It covers all variants of colony count methodsand most probable number estimates.Two approaches, the component (also known as bottom？up or step？by？step) and a modified global (top？down)approach are included.The aim is to specify how values of intralaboratory operational variability and combined uncertainty for finaltest results can be obtained.The procedures are not applicable to methods other than enumeration methods.NOTE 1 Most annexes are normative. However, only the annexes relevant to each case are to be applied. If the choiceis the global approach, then all normative annexes that belong to the component approach can be skipped and vice versa.NOTE 2 Pre-analytical sampling variance at the source is outside the scope of this International Standard, but needsto be addressed in sampling designs and monitoring programmes.NOTE 3 The doubt or uncertainty of decisions based on the use of analytical results whose uncertainty has beenestimated is outside the scope of this International Standard.NOTE 4 The extra？analytical variations observed in proficiency tests and intercalibration schemes are also notdetailed in this International Standard, but it is necessary to take them into consideration in analytical control. The useof intercalibration data in uncertainty estimation offers the possibility for the bias between laboratories to be included(Nordtest Report TR 537[12]).

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