ISO 18395:2005 动物和蔬菜脂肪和油 高性能规格-筛析色谱法（HPSEC） 测量甘油一酯、甘油二酯、甘油三酯和甘油

标准编号：ISO 18395:2005

中文名称：动物和蔬菜脂肪和油 高性能规格-筛析色谱法（HPSEC） 测量甘油一酯、甘油二酯、甘油三酯和甘油

英文名称：Animal and vegetable fats and oils — Determination of monoacylglycerols, diacylglycerols, triacylglycerols and glycerol by high-performance size-exclusion chromatography (HPSEC)

发布日期：2005-04

标准范围

ISO 18395:2005规定了通过高效排阻色谱法测定单酰甘油、二酰甘油和三酰甘油以及游离甘油的方法。适用于以单酰甘油和二酰甘油为主要成分的产品（如乳化剂），其浓度大于10%，并适用于比例小于20%的三酰甘油。该方法不适用于乳脂肪或具有广泛脂肪酸链长度的脂肪和油，因为短脂肪酸的二酰甘油的分子质量低于长脂肪酸的单酰甘油-链脂肪酸。该方法限制了对基于辛酸和癸酸的酰基甘油混合物的适用性。这里，只能测定单酰甘油含量和游离甘油含量。

ISO 18395:2005 specifies a method for the determination of monoacylglycerols, diacylglycerols and triacylglycerols and also free glycerol by high-performance size-exclusion chromatography. It is applicable to products (e.g. emulsifiers) comprising monoacylglycerols and diacylglycerols as main constituents in concentrations greater than 10 %, and to triacylglycerols in a proportion of less than 20 %.The method is not applicable to dairy fats or fats and oils having a wide range of fatty acid chain lengths, since diacylglycerols of short fatty acids have a lower molecular mass than monoacylglycerols of long-chain fatty acids.The method has restricted applicability to acylglycerol mixtures based on caprylic and capric acids. Here, only the monoacylglycerol content and the free glycerol content can be determined.

标准预览图