ISO 22184:2021 牛奶和乳制品 糖含量的测定 脉冲安培检测法高效阴离子交换色谱法 （HPAEC-PAD）

标准编号：ISO 22184:2021

中文名称：牛奶和乳制品 糖含量的测定 脉冲安培检测法高效阴离子交换色谱法 （HPAEC-PAD）

英文名称：Milk and milk products — Determination of the sugar contents — High performance anion exchange chromatography with pulsed amperometric detection method (HPAEC-PAD)

发布日期：2021-02

标准范围

本文件规定了以阿拉伯糖为内标，定量液相色谱法测定各种牛奶和奶制品中特定糖（半乳糖、葡萄糖、果糖、蔗糖、乳糖和麦芽糖）。该方法适用于以下乳制品基质:牛奶、甜炼乳、奶粉、奶酪、乳清粉、婴儿配方奶粉、牛奶甜点和酸奶。该方法不适用于含大豆的乳制品或低乳糖乳制品中乳糖含量低于1 mg/g的测定。应用高效阴离子交换色谱法结合脉冲安培检测（HPAEC-PAD）方法[5][3][4]。用这种方法可以分离出十三种不同的单糖、二糖和三糖:岩藻糖、阿拉伯糖、半乳糖、葡萄糖、果糖、蔗糖、乳糖、乳果糖、麦芽糖、蜜二糖、海藻糖、异麦芽酮糖和麦芽三糖。该方法适用于牛奶和奶制品中天然存在或添加的六种最重要糖的标签。本方法不适用于含糖量低于0.1%的情况。

This document specifies the quantitative liquid chromatographic determination of specific sugars (galactose, glucose, fructose, sucrose, lactose and maltose) in various milk and milk products, applying arabinose as an internal standard.The method is applicable to the following dairy matrices: milk, sweetened condensed milk, milk powder, cheese, whey powder, infant formula, milk dessert and yoghurt.The method does not apply to dairy products containing soy or to the determination of the lactose content in low-lactose milk products at levels below 1 mg/g.A high performance anion exchange chromatography method in combination with pulsed amperometric detection (HPAEC-PAD) method is applied^[5][3][4]. With this method, thirteen different monosaccharides, disaccharides and trisaccharides can be separated: fucose, arabinose, galactose, glucose, fructose, sucrose, lactose, lactulose, maltose, melibiose, trehalose, isomaltulose and maltotriose.The method is applicable to labelling for the six most important sugars that can be present by nature or by addition in milk and milk products. The method does not apply to sugar contents less than 0,1 %.

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