ISO 22174:2005 食品和动物饲料的微生物学-食物致病原体监控用聚合酶连锁反应（PCR） 一般要求和定义

标准编号：ISO 22174:2005

中文名称：食品和动物饲料的微生物学-食物致病原体监控用聚合酶连锁反应（PCR） 一般要求和定义

英文名称：Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions

发布日期：2005-02

标准范围

ISO 22174:2004给出了核酸序列（DNA或RNA）体外扩增的一般要求。本标准适用于使用聚合酶链反应（PCR）对食品和从食品中获得的分离物进行食源性病原体检测ISO 22174:2004中规定的最低要求旨在确保在不同实验室中获得可比较和可再现的结果。ISO 22174:2004适用于食品和饲料基质中或从中分离的食源性病原体，但也适用于其他基质（如环境样品）和非致病微生物的检测。

ISO 22174:2004 gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR)The minimum requirements laid down in ISO 22174:2004 are intended to ensure that comparable and reproducible results are obtained in different laboratories.ISO 22174:2004 has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.

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