ISO 29200:2013 土壤质量 遗传毒性对高等植物的影响评估 蚕豆微核试验

标准编号:ISO 29200:2013

中文名称:土壤质量 遗传毒性对高等植物的影响评估 蚕豆微核试验

英文名称:Soil quality — Assessment of genotoxic effects on higher plants — Vicia faba micronucleus test

发布日期:2013-09

标准范围

ISO 29200:2013的目的是描述一种评估土壤或土壤材料对高等植物蚕豆(蚕豆)次级根的遗传毒性效应(染色体断裂或有丝分裂纺锤体功能障碍)的方法。这种方法允许评估土壤和土壤材料(如堆肥、污泥、废物、施肥物质等)的遗传毒性(对遗传物质的毒性)。可以考虑两种暴露方式:植物直接暴露于与实际遗传毒性潜力相关的土壤(或土壤材料),以及植物暴露于土壤(或土壤材料)的水提取物。这种暴露于渗滤液或洗脱液的最后方式允许检测未吸附到土壤上并且可能转移到水生隔室中的诱变剂。此外,该测试可用于评估化学物质以及对水、流出物等的遗传毒性作用。

The purpose of ISO 29200:2013 is to describe a method for assessing genotoxic effects (chromosome breakage or dysfunction of the mitotic spindle) of soils or soil materials on the secondary roots of a higher plant: Vicia faba (broad bean). This method allows the assessment of genotoxicity (toxicity for genetic material) of soils and soil materials like compost, sludge, waste, fertilizing matters, etc. Two ways of exposure can be considered: a direct exposure of plants to the soil (or soil material) which is relevant for the real genotoxic potential and an exposure of plants to the water extract of the soil (or soil material). This last way of exposure to a leachate or an eluate allows the detection of the mutagens which are not adsorbed to soils and which may be transferred to aquatic compartments. Moreover, this test may be used to evaluate genotoxic effects of chemical substances and to waters, effluents, etc.

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