ISO 11063:2012 土壤质量 从土壤样品中直接提取DNA的方法

标准编号：ISO 11063:2012

中文名称：土壤质量 从土壤样品中直接提取DNA的方法

英文名称：Soil quality — Method to directly extract DNA from soil samples

发布日期：2012-02

标准范围

本国际标准规定了一种从土壤样品中直接提取DNA以分析利用PCR技术研究土壤细菌群落的全球结构和丰度。这种方法主要用于农业和森林土壤。这种方法可能不适用于富含重金属的土壤有机物（如泥炭土）或被有机污染物或重金属严重污染的土壤。从土壤样本中直接提取DNA，可以对土壤的丰富度和结构提供独特的见解微生物群落是评估土壤微生物群落多样性的关键参数。分子的基于PCR（聚合酶链反应）扩增土壤DNA的方法是一种很有前途的方法在不久的将来，该领域将有助于常规工具的开发，以监测土壤环境。使用这种方法的人应该知道，尽管提交DNA提取程序的土壤经过筛选彻底地（2 mm筛孔，5.1中描述的程序），植物残留物仍然可以留在土壤样本中，并且作为结果，微量的植物DNA会污染土壤DNA提取物。

This International Standard specifies a method for direct extraction of DNA from soil samples to analyse theglobal structure and the abundance of soil bacterial communities using PCR-based technologies. This methodis mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich inorganic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.The direct extraction of DNA from soil samples provides unique insight into the richness and structure ofmicrobial communities which are key parameters to estimate the biodiversity of soil microbiota. Molecularapproaches based on PCR (polymerase chain reaction) amplification of soil DNA constitute a promisingdomain and can contribute in the near future to the development of routine tools to monitor the microbiota ofsoil environments.Users of the method ought to be aware that although soil submitted to the DNA extraction procedure is sievedthoroughly (2 mm mesh, procedure described in 5.1), plant residues can still remain in soil samples and, as aresult, traces of plant DNA can contaminate the soil DNA extract.

标准预览图