ISO/TS 20224-10:2024 分子生物标记分析 用实时PCR检测食品和饲料中动物源性物质 第10部分：鸭的DNA检测方法
标准编号：ISO/TS 20224-10:2024
中文名称：分子生物标记分析 用实时PCR检测食品和饲料中动物源性物质 第10部分：鸭的DNA检测方法
英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 10: Duck DNA detection method
发布日期：2024-02
标准范围
本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的鸭特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并可应用于检测来自绿头鸭（Anas platyrhynchos）和斑点嘴鸭（Anas zonorhyncha）的鸭材料。观察到白翅鸭（Asarcornis scutulata）、簇绒鸭（Aythya fuligula）、番鸭（Cairina moschata）和鸳鸯（Aix galericulata）的交叉检测。绿头鸭和番鸭是食用的家养家禽，而斑嘴鸭、白翅鸭、簇绒鸭和鸳鸯是野生鸟类。目的序列是鸭嘴兽品种北京鸭分离物CAU_Pekin_2的部分片段。0CHR1，全基因组鸟枪法序列(即GenBank登录号JACEUM010000001.1)，[1]，其以每个单倍体基因组的单拷贝存在。所提供的用于该靶标的PCR测定的绝对检测限为5？每个反应的副本，用？≥？95？该浓度下的置信度%（LOD95？%）。
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of duck-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of duck material derived from mallard duck (Anas platyrhynchos) and spot-billed duck (Anas zonorhyncha). Cross detection of white-winged duck (Asarcornis scutulata), tufted duck (Aythya fuligula), muscovy duck (Cairina moschata) and Mandarin duck (Aix galericulata) is observed. Mallard duck and muscovy duck are domesticated poultry for food, while spot-billed duck, white-winged duck, tufted duck and Mandarin duck are wild avian.
The target sequence is a partial fragment of the Anas platyrhynchos breed pekin duck isolate CAU_Pekin_2.0 Chr1, whole genome shotgun sequence (i.e. GenBank accession number JACEUM010000001.1),[1] which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).
标准预览图
