ISO 5506:1988 大豆制品.尿素酶活性的测定

标准编号:ISO 5506:1988

中文名称:大豆制品.尿素酶活性的测定

英文名称:Soya bean products — Determination of urease activity

发布日期:1988-08

标准范围

该方法允许检测到产品烹饪不充分。它适用于每克收到的产品中,脲酶活性低于1毫克氮的产品。对于活性更强的产品,试验质量可能会降低。其原理是将研磨试样与缓冲尿素溶液混合,然后将混合物在30℃下保持30分钟,用过量的盐酸溶液中和释放的氨,并用标准体积氢氧化物溶液反滴定。

The method allows inadequate cookingof the products to be detected. It applies to products having a urease activity of less than 1 mg of nitrogen per gram of product as received. For more active products the mass of test may be reduced. The principle consists in mixing of a ground test portion with a buffered urea solution, after keeping the mixture for 30 min at 30 C, neutralization of the ammonia liberated, with an exess of hydrochloric acid solution, and back-titration with standard volumetric hydroxide solution.

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