ISO/TS 21569-5:2016 分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第4部分：FMV启动子（P-FMV）DNA序列检测用实时PCR筛选法

标准编号：ISO/TS 21569-5:2016

中文名称：分子生物标志物分析的水平方法 检测转基因生物体和衍生产品的分析方法 第4部分：FMV启动子（P-FMV）DNA序列检测用实时PCR筛选法

英文名称：Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 5: Real-time PCR based screening method for th

发布日期：2016-11

标准范围

ISO/TS 21569-5:20 16规定了通过实时PCR(聚合酶链式反应)检测转基因(GM)植物中使用的DNA序列的程序。该方法检测玄参花叶病毒34S启动子DNA序列的78个碱基对长区段。该区段在一些转基因植物中表示为FMV启动子(P-FMV)，在其他转基因植物中表示为FMV增强子(E-FMV)。开发并验证了该方法用于分析从食品中提取的DNA。它也可适用于其它产品如饲料和种子的分析。该方法需要从测试样品中提取足够数量和质量的可扩增DNA。通过P-FMV元件特异性方法扩增的DNA序列可以在含有天然存在的玄参花叶病毒DNA的样品中检测到。因此，有必要确认阳性筛查结果。需要使用构建体特异性或事件特异性方法进行进一步分析。

ISO/TS 21569-5:2016 specifies a procedure for the detection of a DNA sequence used in genetically modified (GM) plants by means of a real-time PCR (polymerase chain reaction). The method detects a 78 base pairs long segment of the Figwort mosaic virus 34S promoter DNA sequence. This segment in some GM plants is indicated as FMV promoter (P-FMV) and in other GM plants as FMV enhancer (E-FMV).The method was developed and validated for the analysis of DNA extracted from foodstuffs. It may be suitable also for analysis of other products such as feedstuffs and seeds. The procedure requires the extraction of an adequate quantity and quality of amplifiable DNA from the test sample.The DNA sequence amplified by the P-FMV element-specific method can be detected in samples which contain DNA of the naturally occurring Figwort mosaic virus. For this reason, it is necessary to confirm a positive screening result. Further analyses are required using construct-specific or event specific methods.

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