ISO 11702:2009 动物和植物的脂肪和油 总固醇含量的酶测定

标准编号：ISO 11702:2009

中文名称：动物和植物的脂肪和油 总固醇含量的酶测定

英文名称：Animal and vegetable fats and oils — Enzymatic determination of total sterols content

发布日期：2009-12

标准范围

ISO 11702:2009规定了通过酶染色试验定量测定总甾醇含量的方法。本方法适用于动植物油脂、脂肪食品及相关产品中的游离和酯化甾醇。该测定适用于1g至2g脂肪的样品量。该方法不适用于深色脂肪和油，例如粗棕榈油。这种酶对胆固醇没有特异性，但也会氧化其他3种胆固醇-羟基甾醇。该方法还没有在更高水平的甾醇强化产品中进行测试。

ISO 11702:2009 specifies a method for the quantitative determination of the total sterols content by means of an enzymatic staining test. The method is applicable to free and esterified sterols in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable to sample quantities of 1 g to 2 g of fat.The method is not applicable to dark coloured fats and oils, e.g. crude palm oil. The enzyme is not specific for cholesterol, but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with sterols at higher levels.

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