ISO/TS 20224-8:2022 分子生物标志物分析 用实时PCR检测食品和饲料中的动物源材料 第8部分：火鸡DNA检测方法
标准编号：ISO/TS 20224-8:2022
中文名称：分子生物标志物分析 用实时PCR检测食品和饲料中的动物源材料 第8部分：火鸡DNA检测方法
英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 8: Turkey DNA detection method
发布日期：2022-10
标准范围
本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的火鸡特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并且可以应用于检测来自家养火鸡（Meleagris gallopavo）和野生火鸡（Meleagris ocellata）的火鸡材料。靶序列是黑麦草染色体Z DNA序列(即GenBank登录号NC_015041.2)的部分片段，其以每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR测定的绝对检测限为5？每个反应的副本，用？≥？95？该浓度下的置信度%（LOD95？%）。
This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of turkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of turkey material derived from domestic turkey (Meleagris gallopavo) and wild turkey (Meleagris ocellata).The target sequence is a partial fragment of the Meleagris gallopavo chromosome Z DNA sequence (i.e. GenBank accession number NC_015041.2), which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).
标准预览图
