ISO/TS 20224-6:2020 分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第6部分：马的DNA检测方法

标准编号：ISO/TS 20224-6:2020

中文名称：分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第6部分：马的DNA检测方法

英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 6: Horse DNA detection method

发布日期：2020-07

标准范围

本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的马特异性DNA。它需要从相关基质中提取足够量的PCR扩增DNA，可应用于家马（Equus caballus）、骡（Equus caballus♀× Equus asinus♂）、hinny（Equus caballus♂× Equus asinus♀）和斑马（Equus caballus × Equus simplicidens）的马材料的检测。该测定还检测了普氏原羚马（Equus przewalskii）和斑马（Equus burchellii）。靶序列是Equus caballus分离物Twilight breed纯种28号染色体，EquCab3.0，全基因组鸟枪序列(即GenBank登录号NC_009171.3)[1]，其作为每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR分析具有每个反应5个拷贝的绝对检测限，在该浓度下具有≥95%的可重复性（LOD95%）。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of horse-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of horse material derived from domestic horse (Equus caballus), mule (Equus caballus ♀ × Equus asinus ♂), hinny (Equus caballus ♂ × Equus asinus ♀) and zebroid (Equus caballus × Equus simplicidens). The assay also detects the species Przewalski's horse (Equus przewalskii) and zebra (Equus burchellii).The target sequence is an Equus caballus isolate Twilight breed thoroughbred chromosome 28, EquCab3.0, whole genome shotgun sequence (i.e. GenBank accession number NC_009171.3)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).

标准预览图