ISO 23036-2:2021 食物链微生物学 鱼和水产品中异尖线虫L3幼虫的检测方法 第2部分：人工消化法

标准编号：ISO 23036-2:2021

中文名称：食物链微生物学 鱼和水产品中异尖线虫L3幼虫的检测方法 第2部分：人工消化法

英文名称：Microbiology of the food chain — Methods for the detection of Anisakidae L3 larvae in fish and fishery products — Part 2: Artificial digestion method

发布日期：2021-04

标准范围

本文件规定了一种检测茴香科的方法？L3幼虫常见于海洋和溯河鱼类。该方法适用于鲜鱼和/或冷冻鱼，以及轻度加工的鱼产品，如腌制、腌制或熏制。它也适用于内脏器官，作为目视检查方案的确认方法。人工消化方法[4][5][6]适用于通过估计鱼肌肉组织中寄生虫的数量来量化寄生虫感染，并且当应用于鲜鱼或轻度加工的鱼产品（加工前从不冷冻）时，确定茴香鱼科的生存力？L3，其可以存在。该方法不适用于确定检测到的寄生虫的物种或基因型。通过形态学和/或分子方法进行最终鉴定。

This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.The artificial digestion method^[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

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