ISO 19056-3:2022 显微镜 照明特性的定义和测量 第3部分：入射光荧光显微镜

标准编号：ISO 19056-3:2022

中文名称：显微镜 照明特性的定义和测量 第3部分：入射光荧光显微镜

英文名称：Microscopes — Definition and measurement of illumination properties — Part 3: Incident light fluorescence microscopy with incoherent light sources

发布日期：2022-01

标准范围

本文件规定了入射光荧光显微镜照明亮度、时间稳定性和均匀性的测量程序。当图像平面或中间图像平面适合电子成像设备检测时，均匀性测量仅在图像平面或中间图像平面中定义。本文件定义了如何测量照明亮度、时间稳定性和均匀性，以及如何向用户提供这些信息。注:本范围仅限于电子成像设备和（中间）图像平面。通过目镜进行的视觉观察需要不同的测量程序，因此会导致测量程序的描述不明确。然而，本文件将对视觉观察的一致性给出有用的估计，因为在这种情况下，目镜用于观察中间图像平面（在本文件范围内）。

This document specifies procedures for the measurement of illumination brightness, temporal stability and uniformity for incident light fluorescence microscopy. The measurement for uniformity is defined in image planes or intermediate image planes only, when these planes are suitable for detection by electronic imaging devices.This document defines how illumination brightness, temporal stability and uniformity are measured, and how this information is provided to the user.NOTE      The scope is intentionally limited to electronic imaging devices and (intermediate) image planes. The visual observation by means of eyepieces would require a different measurement procedure and hence result in ambiguities in the description of measurement procedures. Nevertheless, this document will give useful estimates for the uniformity with visual observation as in this case an eyepiece is used to observe an intermediate image plane (which is under the scope of this document).

标准预览图