ISO/TS 22939:2019 土壤质量 在微孔板上用荧光底物测量土壤样品中的酶活性模式

标准编号：ISO/TS 22939:2019

中文名称：土壤质量 在微孔板上用荧光底物测量土壤样品中的酶活性模式

英文名称：Soil quality — Measurement of enzyme activity patterns in soil samples using fluorogenic substrates in micro-well plates

发布日期：2019-08

标准范围

本文件规定了在土壤样品中同时（或不同时）使用荧光底物测量几种酶活性（芳基硫酸酯酶、α-葡萄糖苷酶、β-葡萄糖苷酶、纤维素酶、β-木糖酶、磷酸二酯酶（PDE）、几丁质酶、磷酸单酯酶（PME）、亮氨酸氨基肽酶、丙氨酸氨基肽酶）的方法。土壤酶活性随季节变化，取决于土壤的化学、物理和生物特性。其用于检测有毒化学品的有害影响或其他人为影响，取决于同时比较与试验土壤类似的对照土壤中的酶活性，或使用化学品或处理进行暴露试验。

This document specifies a method for the measurement of several enzyme activities (arylsulfatase, α？glucosidase, β -glucosidase, Cellubisidase, β -Xylosidase, phosphodiesterase (PDE), chitinase, phosphomonoesterase (PME), leucine-aminopeptidase, Alanine-aminopeptidase) simultaneously (or not) using fluorigenic substrates in soil samples. Enzyme activities of soil vary seasonally and depend on the chemical, physical and biological characteristics of soil. Its application for the detection of harmful effects of toxic chemicals or other anthropogenic impacts depends on the simultaneous comparison of enzyme activities in a control soil similar to the test soil, or on exposure tests with chemicals or treatments.

标准预览图