ISO 21528-1:2004 食品和动物饲料的微生物学-观察和计数肠杆菌科用水平线法-第1部分: 采用MPN(最大可能值)技术配合预浓缩技术的发现和计数

标准编号:ISO 21528-1:2004

中文名称:食品和动物饲料的微生物学-观察和计数肠杆菌科用水平线法-第1部分: 采用MPN(最大可能值)技术配合预浓缩技术的发现和计数

英文名称:Microbiology of food and animal feeding stuffs — Horizontal methods for the detection and enumeration of Enterobacteriaceae — Part 1: Detection and enumeration by MPN technique with pre-enrichment

发布日期:2004-08

标准范围

ISO 21528-1:2004规定了一种检测肠杆菌科的预富集方法。适用于人类消费和动物饲养的产品,以及食品生产和食品处理领域的环境样品。在37°C的液体培养基中培养后,通过计算最可能数(MPN)进行计数。当所寻求的微生物预计在浓缩前需要复苏,且所寻求的数量预计在每毫升或每克试样1到100的范围内时,本方法适用。ISO 21528-1:2004的适用性受到了限制,因为该方法易受很大程度变化的影响

ISO 21528-1:2004 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium.This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability

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