ISO 6888-2:2021 食品和动物饲料原料的微生物学 阳极凝固酶葡萄球菌落计数方法 第二部分：使用兔子血桨纤维蛋白质琼脂介质技术.

标准编号：ISO 6888-2:2021

中文名称：食品和动物饲料原料的微生物学 阳极凝固酶葡萄球菌落计数方法 第二部分：使用兔子血桨纤维蛋白质琼脂介质技术.

英文名称：Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 2: Method using rabbit plasma fibrinogen aga

发布日期：2021-08

标准范围

本文件规定了凝固酶阳性葡萄球菌计数的水平方法，方法是在34°C至38°C的需氧培养后，在固体培养基（兔血浆纤维蛋白原琼脂培养基）上计数菌落（见参考文献[10]）。本文件适用于:-？供人类消费的产品；-？用于动物喂养的产品；-？食品和饲料生产和处理领域的环境样本；-？初级生产阶段的样品。这种水平方法最初是为检测食物链中的所有样本而开发的。由于食物链中的产品种类繁多，这种横向方法可能并不适用于所有产品的每个细节。尽管如此，预计所需的修改将被最小化，以便它们不会导致与该水平方法的重大偏差。根据本文件发布时的可用信息，本方法不适用于（完全）检测发酵产品或其他含有基于葡萄球菌属（如木糖）的工艺菌群的产品（如由生牛奶和某些生肉制品制成的奶酪），可能受到以下污染:-？在Baird-Parker琼脂培养基上形成非典型菌落的葡萄球菌；-？背景植物群，可能会掩盖正在寻找的殖民地。然而，ISO？6888-1和本文件均被赋予同等地位。

This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]).This document is applicable to:—    products intended for human consumption;—    products intended for animal feeding;—    environmental samples in the area of food and feed production and handling;—    samples from the primary production stage.This horizontal method was originally developed for the examination of all samples belonging to the food chain.Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.

Based on the information available at the time of publication of this document, this method is particularly suitable for the examination of fermented products or other products containing technological microbiota based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:

—     staphylococci forming atypical colonies on a Baird-Parker agar medium;

—     background microbiota that can obscure the colonies being sought.Nevertheless, both ISO 6888-1 and this document are given equivalent status.

标准预览图