ISO 6888-1:2021 食品和动物饲料原料的微生物学-阳极凝固酶葡萄球菌落计数水平方法-第一部分：使用磷酸盐琼脂介质技术

标准编号：ISO 6888-1:2021

中文名称：食品和动物饲料原料的微生物学-阳极凝固酶葡萄球菌落计数水平方法-第一部分：使用磷酸盐琼脂介质技术

英文名称：Microbiology of the food chain — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Part 1: Method using Baird-Parker agar medium

发布日期：2021-08

标准范围

该文件规定了一种用于计数凝固酶阳性葡萄球菌的水平方法，该方法通过计数在34℃有氧培养后在固体培养基（Baird-Parker培养基）[10]上获得的菌落？℃至38℃？C和凝固酶确认。本文件适用于:—？供人类消费的产品；—？用于动物喂养的产品；—？食品和饲料生产、处理和—？初级生产阶段的样品。这种水平方法最初是为检查属于食物链的所有样品而开发的。由于食物链中的产品种类繁多，这种横向方法可能并不适合所有产品的每个细节。然而，期望所需的修改被最小化，使得它们不会导致与该水平方法的显著偏差。根据本文件发布时可获得的信息，认为该方法不（完全）适用于检测发酵产品或其他含有基于葡萄球菌属（如木糖葡萄球菌）的技术菌群的产品（如由原料奶和某些生肉产品制成的奶酪），这些菌群可能被以下物质污染:—？在Baird-Parker琼脂培养基上形成非典型菌落的葡萄球菌；—？可以掩盖正在寻找的菌落的背景菌群。然而，无论是本文件还是ISO？6888-2被赋予同等地位。

This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (Baird-Parker medium)^[10] after aerobic incubation at 34 °C to 38 °C and coagulase confirmation.This document is applicable to:—    products intended for human consumption;—    products intended for animal feeding;—    environmental samples in the area of food and feed production, handling, and—    samples from the primary production stage.This horizontal method was originally developed for the examination of all samples belonging to the food chain.Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.Based on the information available at the time of publication of this document, this method is not considered to be (fully) suited to the examination of fermented products or other products containing technological flora based on Staphylococcus spp (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:—    staphylococci forming atypical colonies on a Baird-Parker agar medium;—    background flora that can obscure the colonies being sought.Nevertheless, both this document and ISO 6888-2 are given equivalent status.

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