ISO 16266-2:2018 水质 铜绿假单胞菌的检测和计数 第2部分：最大概率法

标准编号：ISO 16266-2:2018

中文名称：水质 铜绿假单胞菌的检测和计数 第2部分：最大概率法

英文名称：Water quality — Detection and enumeration of Pseudomonas aeruginosa — Part 2: Most probable number method

发布日期：2018-07

标准范围

本文件规定了水中铜绿假单胞菌的计数方法。该方法基于目标生物在液体培养基中的生长和通过参考MPN表计算生物的最可能数量(MPN)。本文件适用于各种类型的水。例如，医院用水、饮用水和非饮用水？供人饮用的碳酸瓶装水、地下水、游泳池和水疗池水，包括异养细菌背景计数高的水。本文件不适用于该方法未经验证的碳酸瓶装水、调味瓶装水、冷却塔水或海水。因此，这些水域不在本文件的范围之内。实验室可以通过在使用前对该方法的性能进行适当验证，对这些基质采用本文件中介绍的方法。该测试基于细菌酶检测技术，该技术通过水解7？氨基？4？特殊试剂中存在的甲基香豆素氨肽酶底物。铜绿假单胞菌细胞利用试剂中存在的丰富的氨基酸、维生素和其他营养物质快速生长和繁殖。活跃生长的铜绿假单胞菌菌株有一种酶可以切割7？淀粉？香豆素氨肽酶底物释放在紫外线(UV)下发出荧光的产物。本文件中描述的测试在24小时内提供了确认的结果，无需进一步确认阳性孔。

This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The method is based on the growth of target organisms in a liquid medium and calculation of the most probable number (MPN) of organisms by reference to MPN tables.This document is applicable to a range of types of water. For example, hospital waters, drinking water and non？carbonated bottled waters intended for human consumption, groundwater, swimming pool and spa pool waters including those containing high background counts of heterotrophic bacteria.This document does not apply to carbonated bottled waters, flavoured bottle waters, cooling tower waters or marine waters, for which the method has not been validated. These waters are, therefore, outside the scope of this document. Laboratories can employ the method presented in this document for these matrices by undertaking appropriate validation of performance of this method prior to use.The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a 7？amino？4？methylcoumarin aminopeptidase substrate present in a special reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the 7？amido？coumarin aminopeptidase substrate releasing a product which fluoresces under ultraviolet (UV) light. The test described in this document provides a confirmed result within 24 h with no requirement for further confirmation of positive wells.

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