ISO/TS 12869:2019 水质 利用定量聚合酶链反应（QPCR）实现浓缩和基因扩增的方式检测和量化军团菌属和/或嗜肺军团菌

标准编号：ISO/TS 12869:2019

中文名称：水质 利用定量聚合酶链反应（QPCR）实现浓缩和基因扩增的方式检测和量化军团菌属和/或嗜肺军团菌

英文名称：Water quality — Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)

发布日期：2019-04

标准范围

本文件规定了使用定量聚合酶链反应（qPCR）检测和定量军团菌属和嗜肺军团菌的方法。它规定了一般方法要求、性能评估要求和质量控制要求。本文件中规定的技术细节仅供参考。符合性能要求的任何其他技术解决方案都是合适的。注1:性能要求见第9条。本文件适用于所有类型水（热水或冷水、冷却塔水等）的细菌学调查，除非悬浮物和/或伴随菌群的性质和/或含量干扰测定。这种干扰会对检测限和定量限产生不利影响。注2:验证要求见9。7.结果以每升样本中军团菌属和/或嗜肺军团菌的基因组单位数表示。本文件中描述的方法适用于所有类型的水。然而，一些添加剂，例如用于水处理的化学品，可能会干扰和/或影响该方法的灵敏度。qPCR方法不提供有关军团菌生理状态的任何信息。

This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable.NOTE 1 For performance requirements, see Clause 9.This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.NOTE 2 For validation requirements, see 9.7.The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.The qPCR methods do not give any information about the physiological state of the Legionella.

标准预览图