ISO/TS 4985:2023 乳和乳制品 碱性磷酸酶活性的测定 荧光微孔板法

标准编号：ISO/TS 4985:2023

中文名称：乳和乳制品 碱性磷酸酶活性的测定 荧光微孔板法

英文名称：Milk and milk products — Determination of alkaline phosphatase activity — Fluorimetric microplate method

发布日期：2023-07

标准范围

本文件规定了一种荧光微孔板法，用于测定生的和热处理的全脂牛奶、半脱脂牛奶、脱脂牛奶、奶油、调味牛奶和奶酪中的碱性磷酸酶（ALP，EC 3.1.3.1）[5]活性。该方法适用于来自奶牛、绵羊和山羊的奶和奶基饮料。虽然该方法没有在来自其他物种的奶中进行测试，但它也可以适用于来自具有与牛奶、绵羊或山羊奶相似组成的其他物种的奶，例如来自水牛和骆驼的奶。它也适用于重构后的奶粉以及软、半硬和硬干酪，条件是模具仅在干酪的表面上而不在内部部分中(例如蓝纹干酪)。对于大型硬干酪，特定的取样条件适用（见条款？7）.笔记？此方法改编自参考文献？[6].

This document specifies a fluorimetric microplate method for the determination of alkaline phosphatase (ALP, EC 3.1.3.1)^[5] activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk, cream, flavoured milks and cheeses.

This method is applicable to milk and milk-based drinks from cows, sheep and goats. Although the method was not tested in milk from other species, it can also be applicable to milk from other species with a similar composition to cow, sheep or goat milk, such as milk from buffalo and camelids. It is also applicable to milk powder after reconstitution and soft, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (e.g. blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7).

NOTE            This method was adapted from Reference [6].

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