ISO 5542:1984 牛奶.蛋白质含量的测定.酰胺黑染色结合法（常规法）

标准编号：ISO 5542:1984

中文名称：牛奶.蛋白质含量的测定.酰胺黑染色结合法（常规法）

英文名称：Milk — Determination of protein content — Amido black dye-binding method (Routine method)

发布日期：1984-05

标准范围

适用于未经加工或热加工或机械加工的全脂牛奶、部分脱脂牛奶和脱脂牛奶，前提是样品状况良好。在某些情况下，它也适用于保存的样品。测定原理包括向试验部分添加氨基黑溶液，形成不溶性染料-蛋白质复合物，通过离心或过滤去除，然后根据含有过量染料的所得溶液的吸光度测定蛋白质含量。

Is applicable to raw or thermally or mechanically processed whole milk, partly skimmed and skimmed milk provided that the samples are in good condition. In some cases it can also be applied to preserved samples. The principle of determination consists in adding amido black solution to a test portion resulting in the formation of an insoluble dye-protein complex which is removed by centrifuging or filtration, followed by determination of the protein content from the absorbance of the resulting solution containing an excess of dye.

标准预览图