ISO/TS 20224-3:2020 分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第3部分：猪的DNA检测方法

标准编号：ISO/TS 20224-3:2020

中文名称：分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第3部分：猪的DNA检测方法

英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 3: Porcine DNA detection method

发布日期：2020-07

标准范围

本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食品和饲料的猪特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并且可以应用于检测来自猪（Sus scrofa domesticus）和野猪（Sus scrofa）的猪材料。靶序列是猪β肌动蛋白(ACTB)基因的部分片段，部分cds。（即GenBank登录号DQ452569.1）[1]，其以每个单倍体基因组的单拷贝存在。所提供的用于该靶标的PCR分析具有每个反应5个拷贝的绝对检测限，在该浓度下具有≥95%的可重复性（LOD95%）。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of porcine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of porcine material derived from pig (Sus scrofa domesticus) and wild boar (Sus scrofa).The target sequence is a partial fragment of the Sus scrofa beta actin (ACTB) gene, partial cds. (i.e. GenBank accession number DQ452569.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).

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