ISO 22119:2011 食品和动物饲料的微生物学 食物传播的病原体的检测用实时聚合酶链式反应（PCR） 一般要求和定义

标准编号：ISO 22119:2011

中文名称：食品和动物饲料的微生物学 食物传播的病原体的检测用实时聚合酶链式反应（PCR） 一般要求和定义

英文名称：Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions

发布日期：2011-07

标准范围

ISO 22119:2010定义了使用聚合酶链反应（PCR）检测食品中的食源性病原体和从中获得的分离物的术语。ISO 22119:2010规定了通过实时PCR扩增和检测核酸序列（反转录后的DNA或RNA）的要求。ISO 22119:2010中规定的最低要求为单个实验室内和不同实验室之间的可比和可再现结果提供了基础。例如，ISO 22119:2010也适用于食品检测-环境样本和动物饲料中的病原体。

ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories.ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.

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