ISO 3594:1976 乳脂.用甾醇气一液相色谱法检验植物油脂(参比法)

标准编号:ISO 3594:1976

中文名称:乳脂.用甾醇气一液相色谱法检验植物油脂(参比法)

英文名称:Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)

发布日期:1976-12

标准范围

该方法包括制备甾醇地高辛并溶解在甲酰胺和二甲基甲酰胺的混合物中,用戊烷萃取释放的甾醇,使用合适的气相色谱仪分离甾醇。如果在色谱图上获得了β-谷甾醇保留时间的峰值,则表明样品中存在植物脂肪。其他植物甾醇的峰值可能支持这一结论。

The method consists in preparing of sterol digitonides and dissolving in a mixture of formamide and dimethylformamide, extraction of the liberated sterols with pentane, separation of the sterols using a suitable gas chromatograph. If, on the chromatogram, a peak with the retention time of beta-sitosterol is obtained, the presence of vegetable fat in the sample is demonstrated. Peaks of other phytosterols may support this conclusion.

标准预览图


立即下载标准文件