ISO/TS 20224-2:2020 分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第2部分：绵羊DNA检测方法

标准编号：ISO/TS 20224-2:2020

中文名称：分子生物标记物分析 用实时PCR法检测食品和饲料中的动物源性材料 第2部分：绵羊DNA检测方法

英文名称：Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 2: Ovine DNA detection method

发布日期：2020-07

标准范围

本文件规定了一种实时聚合酶链式反应（real-time PCR）方法，用于定性检测来自食物和饲料的绵羊特异性DNA。它需要从相关基质中提取足够量的PCR可扩增DNA，并且可以应用于检测源自绵羊（Ovis aries）的绵羊材料。靶序列是绵羊核催乳素受体短型mRNA基因（PRLR）的部分片段（即GenBank登录号AF041979.1）[1]，其以每个单倍体基因组的单个拷贝存在。所提供的用于该靶标的PCR分析具有每个反应5个拷贝的绝对检测限，在该浓度下具有≥95%的可重复性（LOD95%）。

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep (Ovis aries).The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene (PRLR) (i.e. GenBank accession number AF041979.1)^[1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % replicability at this concentration (LOD_{95 %}).

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