ISO/TS 21569-3:2020 分子生物标志物分析的水平方法 检测转基因生物体及其衍生产品的分析方法 第3部分：筛选基因变异后生物用检测转基因（P35S）实时性聚合酶链反应检测方法

标准编号：ISO/TS 21569-3:2020

中文名称：分子生物标志物分析的水平方法 检测转基因生物体及其衍生产品的分析方法 第3部分：筛选基因变异后生物用检测转基因（P35S）实时性聚合酶链反应检测方法

英文名称：Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for

发布日期：2020-06

标准范围

该文献描述了用于检测来自花椰菜花叶病毒的35S启动子(P35S)和来自绿色产色链霉菌的修饰的磷丝菌素-乙酰转移酶基因(pat)之间的DNA转换序列的方法。P35S-pat构建体经常存在于对含膦丝菌素的除草剂具有耐受性的转基因植物中。P35S-pat构建体特异性方法基于实时PCR，可用于定性和定量筛选目的。为了识别和量化特定事件，可以进行后续分析。本文件适用于从食品中提取的DNA的分析。它还可以适用于从其他产品如饲料和种子中提取的DNA的分析。该方法的应用需要从相关基质中提取足够数量和质量的可扩增DNA。

This document describes a procedure for the detection of the DNA transition sequence between the 35S promotor (P35S) from Cauliflower mosaic virus and a modified phoshinothricin-acetyltransferase gene (pat) from Streptomyces viridochromogenes. The P35S-pat construct is frequently found in genetically modified plants with tolerance for phosphinothricin-containing herbicides. The P35S-pat construct specific method is based on a real-time PCR and can be used for qualitative and quantitative screening purposes. For identification and quantification of a specific event, a follow-up analysis can be carried out.This document is applicable to the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate quantity and quality of amplifiable DNA from the relevant matrix.

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