ISO 20838:2006 食品及饲料的微生物学-食品内含病原体（物）检测用聚合连锁反应-定性方法的放大和检测的要求

标准编号：ISO 20838:2006

中文名称：食品及饲料的微生物学-食品内含病原体（物）检测用聚合连锁反应-定性方法的放大和检测的要求

英文名称：Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for amplification and detection for qualitative methods

发布日期：2006-04

标准范围

ISO 20838:2006提供了使用聚合酶链反应（PCR）检测食源性病原体的定性方法的总体框架。它涵盖了目标核酸序列特异性扩增的一般要求，以及检测和确认扩增核酸序列的身份。ISO 20838:2006中描述的指南、最低要求和性能特征旨在确保在不同实验室获得可比较和可再现的结果。ISO 20838:2006适用于食品和饲料基质中或从中分离的食源性病原体，但也可应用于其他基质，例如环境样品，或用于检测正在调查的其他微生物。

ISO 20838:2006 provides the overall framework for qualitative methods for the detection of food-borne pathogens using the polymerase chain reaction (PCR).It covers the general requirements for the specific amplification of target nucleic acid sequences and the detection and confirmation of the identity of the amplified nucleic acid sequence.Guidelines, minimum requirements and performance characteristics described in ISO 20838:2006 are intended to ensure that comparable and reproducible results are obtained in different laboratories.ISO 20838:2006 has been established for food-borne pathogens in or isolated from food and feed matrices, but can also be applied to other matrices, for example environmental samples, or to the detection of other microorganisms under investigation.

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