ISO 18363-4:2021 动植物脂肪和油 用GC/MS测定脂肪酸结合的氯丙二醇（MCPD）和缩水甘油 第4部分：快速碱性酯交换法和用GC-MS/MS测定2-MCPD，3-MCPD和缩水甘油

标准编号：ISO 18363-4:2021

中文名称：动植物脂肪和油 用GC/MS测定脂肪酸结合的氯丙二醇（MCPD）和缩水甘油 第4部分：快速碱性酯交换法和用GC-MS/MS测定2-MCPD，3-MCPD和缩水甘油

英文名称：Animal and vegetable fats and oils — Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS — Part 4: Method using fast alkaline transesterification and measurement for 2-M

发布日期：2021-08

标准范围

本文件规定了一种快速测定2-MCPD酯（结合2-MCPD）、3-MCPD酯（结合3-MCPD）和缩水甘油酯（结合缩水甘油）的方法，该方法基于碱性催化酯裂解和裂解（游离）分析物在GC-MS/MS分析前与苯硼酸（PBA）衍生。通过添加同位素标记的酯结合3-MCPD，可以纠正缩水甘油酯高估，从而在操作过程中量化3-MCPD诱导的缩水甘油。该方法适用于固体和液体脂肪和油。本文件也适用于动物脂肪和用过的煎炸油和脂肪，但这些基质不包括在验证中。对于所有三种分析物，定量限（LOQ）为0.1？mg/kg，检测限（LOD）为0.03？mg/kg。本文件不包括牛奶和奶制品（或来自牛奶和奶制品的脂肪）、婴儿配方奶粉、乳化剂、游离脂肪酸和其他脂肪及油衍生基质。

This document specifies a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3‐MCPD esters (bound 3‐MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. Glycidyl ester overestimation is corrected by addition of an isotopic labelled ester bound 3-MCPD which allows the quantification of 3-MCPD induced glycidol during the procedure.This method is applicable to solid and liquid fats and oils. This document also applies to animal fats and used frying oils and fats, but these matrices were not included in the validation. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg.Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats- and oils-derived matrices are excluded from the scope of this document.

标准预览图