ISO/TS 20131-1:2018 土壤质量 土壤反硝化的简易实验室评估、N₂O排放的过程源 第1部分：土壤反硝化酶活性

标准编号：ISO/TS 20131-1:2018

中文名称：土壤质量 土壤反硝化的简易实验室评估、N₂O排放的过程源 第1部分：土壤反硝化酶活性

英文名称：Soil quality — Easy laboratory assessments of soil denitrification, a process source of N2O emissions — Part 1: Soil denitrifying enzymes activities

发布日期：2018-07

标准范围

本文件规定了表征土壤中反硝化酶活性的实验室试验。它全面描述了硝酸盐形式转化为氧化亚氮和二氮形式的过程。该方法由参考文献[5]首次提出，首字母缩写为DEA，用于反硝化酶活性。这是许多科学研究中使用的标准化技术。DEA估计了在脱氮过程的最佳基质（硝酸盐和碳源）和环境（厌氧、受控温度）条件下培养的新鲜土壤样品的脱氮过程。氯霉素的使用阻碍了酶的从头合成。DEA被认为代表了采样时土壤样本中反硝化酶池的大小。该试验是在实验室中对按地块规模采集的筛分样品进行的。它可以在全年取样的所有类型的土壤上进行。

This document specifies a laboratory test for characterizing the denitrifying enzyme activities in soils^[5]. It globally characterizes the transformation of the nitrate form to the nitrous oxide and dinitrogen forms. This method was first proposed by Reference [5] with the acronym DEA for Denitrifying Enzyme Activities. It is a standardized technique used in numerous scientific studies.DEA estimates the process of denitrification of fresh soil samples incubated under optimal conditions of substrates (nitrate and carbon sources) and environment (anaerobiosis, controlled temperature) for the denitrification process. The de novo enzyme synthesis is blocked by the use of chloramphenicol. DEA is believed to represent the size of the denitrifying enzyme pool present in the soil sample at the time of sample collection.This test is performed in laboratory on a composite of sieved samples collected at the plot scale. It can be performed on all types of soils sampled all over the year.

标准预览图